Our laboratory works in the area of chemical biology and chemical proteomics. We develop and apply small molecule tools that covalently modify proteins according to a certain trait. We particularly focus on molecules named activity-based probes. These react with active enzymes within a whole proteome and enable a read-out by SDS-PAGE, mass spectrometry, fluorescence spectroscopy or microscopy. Besides probe development, we focus on several protease families including the intramembrane rhomboid proteases. Using chemical techniques, we aim to get insight into the biochemical mechanism and biological function, and elucidate whether these enzymes may be used as a future target for drug development. Our approach is interdisciplinary in nature and uses synthetic organic chemistry, biochemistry, molecular biology, cell culture and proteomics. For more information, check out our research and publication pages.

Of course, we always look out for talented students and post-docs who are interested in joining our lab. Please contact Steven Verhelst for more information.

Covalent chemical probes